Effects of NFI family members on the expression of KLF4 and E-cadherin. (A) Expression of HA, KLF4, and E-cadherin proteins was analyzed by western blotting (left panel) and quantified (right panel) in MCF7 cells transfected with HA-tagged-NFI-A, −NFI-B, −NFI-C, or -NFI-X expressing constructs. (B) The transcriptional activity of the E-cadherin promoters was evaluated by luciferase assay in MCF7 cells transfected with NFI-A-, NFI-B-, NFI-C-, or NFI-X-expressing constructs. The data are presented as the mean ± SD of triplicate experiments. * denotes values significantly different from the control (P < 0.01).