Figure 5

Gene expression analysis shows that S100A2 expression in MCF-7 cells is modulated by the activity of IFN-positive CAFs. (A) A 2 × 2 × 2 SAM analysis was carried out to compare MCF-7 s under three different conditions: MCF-7 monocultured with only the IgG₁ antibody, MCF-7 co-cultured with two IFN-positive CAFs and the IgG₁ antibody, and MCF-7 co-cultured with the same two IFN-positive CAFs in addition to the neutralizing IFN-β monoclonal antibody. 995 modulated genes and corresponding clustering are displayed. (B) The 19 transcripts that are significantly different between the second condition and both the first and third conditions are magnified. S100A2 (red arrow) is highlighted. (C)S100A2’s differential mRNA expression was confirmed by Q-RT-PCR. An ANOVA analysis followed by a Bonferonni multiple comparison test was performed to show that S100A2 expression rose when the IFN-β neutralizing antibody was added to the IFN-positive co-culture. (D) The Kaplan Meir analysis on the NKI295 cohort when S100A2 expression is low (left) shows that IFN-positive patients (red) have a significantly shorter survival duration when compared to IFN-negative (blue) patients. Conversely, when S100A2 expression is high (plot on right), the IFN-positive and negative groups have statistically indistinguishable survival profiles.