Figure 3

MCF-7-CAF co-culture phenotype. Three types of fibroblasts were involved in co-cultures with the MCF-7 breast cancer cell line. Normal breast fibrobasts are denoted in green, IFN-negative CAFs in blue and IFN-positive CAFs in red. All experiments were performed in triplicate. A single 120 hour time point is shown. Statistical differences were ascertained by an analysis of variance. Double asterisks represent samples that were deemed to be significantly different (p < 0.01) from the MCF-7 grown in mono-culture (post-hoc test: Dunnett’s multiple comparison test). Five of six CAFs showed significant growth promotion of the MCF-7 breast cancer cell line in this model.