Figure 2

Modulation of expression of miRNA-141 or miRNA-200b in CRL 2749 and HTB9 leaded to changes of MMPs expression and the invasive potential. Transduced and untransduced cells were plated on the top chamber for 48 h, before photos were taken of invaded cells. Results were expressed as the number of invaded cells relative to untransduced control cells, as determined from three independent experiments (A). *P < 0.05 compared with untransduced control. Gelatine zymography was performed with cell supernatants harvested after 24 h of cultivation. Gelatinolytic activity was attributed to the activity of MMP-2 and MMP-9 (B). Cell lysates of untransduced and transduced cells were subjected to immunoblot analysis for MMP-2, MMP-9 and GAPDH (B).