Figure 1

Expression of miRNA 200 family in bladder cell lines and in vitro lentiviral transduction in bladder cell lines. Expression (±SD) of miRNA-200a, −200b, −200c, −141 and 429 in a panel of 5 bladder cancer cell lines was determined by TaqMan miRNA qRT-PCR assays (A). Mean of triplicate RT-PCR assays. Expression was normalized to U6 (2^−ΔCt). Stable lentiviral transduction of pri-miRNA-141 and -200b into CRL 1749 cells, and miRNA-141-sponge and miRNA-200b-sponge into HTB9 cells was performed. Transduction efficiency was determined by fluorescent microscopy after transduction with lentivector encoding green fluorescence protein. Over 80% of transduced cells showed green fluorescent signals. Magnification was 200× (B). The expression levels of miR-141 or -200b were determined by RT-PCR (C and D). The expression of miR-141/200b was significantly overexpressed in CRL 1749 pri-miR-141/200b cells, while greatly repressed in HTB9 miR-141/200b-sponge cells when compared with respective untransduced and empty lentivirus control cells (**P < 0.01). CRL 1749 pri-miR-141/200b or HTB9 miR-141/200b-sponge cells referred to cells stably transduced with lentivirus encoding pri-miR-141/200b or miR-141/200b -sponge.