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Figure 5 | BMC Cancer

Figure 5

From: ADAM12-L is a direct target of the miR-29 and miR-200 families in breast cancer

Figure 5

Relationship between endogenous miR-29b, miR-200c, andADAM12-Lin breast tumors and breast cancer cell lines. (A) Correlation between ADAM12-L and miRNA levels in a set of 100 human breast tumors profiled with the Agilent Whole Human Genome Microarray 4×44K G4112F and the Agilent Human miRNA Microarray 2.0 G4470B, based on ref. [44]. The expression data were retrieved from GEO:GSE19536. Expression values of ADAM12-L were based on the A_23_P202327 probe. The expression values were median-centered for all tumors. Pearson r and P values are shown for each comparison. (B) SUM102PT and SUM149PT cells were transfected with miRNA hairpin inhibitors targeting miR-29b or miR-200c, or with hairpin inhibitor control. ADAM12-L mRNA levels were measured by qRT-PCR and normalized to β-ACTIN. Fold changes in miRNA inhibitor-transfected cells versus control cells were calculated. Graphs represent average values obtained in three independent experiments ± SEM. Statistical significance determined by one-sample t tests. *P < 0.05, **P < 0.01.

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