Figure 1

Pattern of expression ofADAM12-L,ADAM12-S, and miRNAs in breast cancer cell lines. (A) Discrepancy between ADAM12-L and ADAM12-S levels in a panel of human breast cancer cells profiled with the Agilent 4×44K UNC custom microarray platform, based on ref. [39]. The expression data were retrieved from GEO:GSE50470. Expression values of ADAM12-L were calculated as the average readouts for three probes (A_23_P202327, NM_003474_2_4965, and NM_003474_2_4854). Expression values of ADAM12-S are based on the A_23_P350512 probe. (B) Discrepancy between ADAM12-L and ADAM12-S expression levels in a panel of human breast cancer cells profiled with the Affymetrix HG-U133A platform, based on ref. [40]. The expression data were retrieved from ArrayExpress, accession number E-TABM-157. (C) Predicted miR-29b/c, miR-30b/d, and miR-200b/c target sites in the human ADAM12-L 3′UTR, based on TargetScan Release 6.2. (D) miR-29b, miR-29c, miR-30d, miR-200b, and miR-200c levels in a panel of breast cancer cell lines, based on ref. [38]. Expression data for miR-30b were not available. Downloaded data were log₂-transformed, median-centered and Z scores were calculated. In A, B, and D, each colored square in the heatmaps represents the relative transcript abundance, in log₂ space. Expression values were median-centered across all cell lines.