Representative immunohistochemistry in the primary tumor tissues for anti-phosphorylated Akt (Ser-473), phosphorylated Akt (Thr-308), and phosphorylated S6 ribosomal protein (Ser-235/236) antibodies. Some of tumor cells showed brown staining for anti-pAkt (Ser-473) antibody (arrows), while glomerulus and renal tubules did not (A. ×100). Many of tumor cells showed moderate to strong brown staining in a membrane and cytoplasm in RCC for anti-pAkt (Ser-473) antibody (B. ×200), for anti-pAkt (Thr-308) antibody (C. ×200), and for anti-pS6 antibody (D. ×200).