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Table 1 List of primers used in RT-PCR for expression of stem cell transcription factors

From: Human non-small cell lung cancer expresses putative cancer stem cell markers and exhibits the transcriptomic profile of multipotent cells

Genes

Forward primer

Reverse primer

Sox2

GGTTACCTCTTCCTCCCACTCAG

TCACATGTGCGACAGGGGCAG

Klf4

CACCATGGACCCGGGCGTGGCTGCCAGAAA

AAGCTGACTTGCTGGGAACTTGACC

c-Myc

CAGAGGAGGAACGAGCTGAAGCGC

TTATGCACCAGAGTTTCGAAGCTGTTCG

Nanog

AGGGTCTGCTACTGAGATGCTCTG

CAACCACTGGTTTTTCTGCCACCG

Oct 3/4

CTGAGGGCCAGGCAGGAGCACGAG

CTGTAGGGAGGGCTTCGGGCACTT

GAPDH

CCGCATCTTCTTGTGCAGTG

CTGTGGTCATGAGCCCTTCC

Sox2

GGTTACCTCTTCCTCCCACTCAG

TCACATGTGCGACAGGGGCAG

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BMC Cancer

ISSN: 1471-2407

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