Figure 7

Microarray analysis and validation. (A) Volcano plot of differentially expressed genes obtained from microarray analysis. The red coloured dots represent the differentially expressed genes (fold change > 2, p-value < 0.05). (B) Validation of the microarray data by RT-PCR for six genes in putative CSCs. Up-regulation of three genes (CDH2, AKT3, and EPHX1) and down-regulation of three genes (ITGA3, THBS1, and LAMC2) were observed in all cells. (C) Results of the correlation coefficient test between data from the microarray (log2) and RT-PCR (relative to GAPDH) assays for the genes subjected to the validation study. The X-axis shows the log transformed array data and the Y-axis shows the RT-PCR data for each sample.