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Figure 2 | BMC Cancer

Figure 2

From: Role of SMC1A overexpression as a predictor of poor prognosis in late stage colorectal cancer

Figure 2

Functional analysis of SMC1A by shRNA in CRC cells. (A) Expression analysis of SMC1A in five different CRC cell lines by real-time PCR (upper panel) and western blotting (lower panel). (B) Expression analysis of SMC1A in RKO cells after Lv-shSMC1A infection by real-time PCR (upper panel) and western blotting (lower panel). (C) Expression analysis of SMC1A in SW480 cells after Lv-shSMC1A infection by real-time PCR (upper panel) and western blotting (lower panel). β-actin gene and GAPDH protein were used as internal controls. The proliferation levels of RKO (D, E) and SW480 (F) cells after Lv-shSMC1A infection analyzed by the MTT assay. The number of colonies in RKO (G, H) and SW480 (I) cells after Lv-shSMC1A infection analyzed by the colony formation assay. (J) The percentages of RKO cells using three different treatments in different phases (left panel) and the sub-G1 phase (right panel) of the cell cycle. (K) RKO cells stained with Annexin V and 7-AAD analyzed using flow cytometer (left panel). Q1, Annexin V/7-AAD+; Q2, Annexin V+/7-AAD+; Q3, Annexin V/7-AAD; Q4, Annexin V+/7-AAD. Quantification of the percentage of early apoptotic cells and late apoptotic cells (right panel). *p < 0.05, **p < 0.01, ***p < 0.001 in comparison with non-silencing shRNA infected control.

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