SMC1A staining is demonstrated mainly at the membrane of tumor cells. Positive cells were stained brown. The intensity of SMC1A staining was assigned the following scores: negative = − (A), slightly positive = + (B), moderately positive = ++ (C) and strongly positive = +++ (D). Examples of representative immunohistochemistry results are shown. Scale bars = 50 μm. Kaplan-Meier analysis of (E) overall survival (p = 0.008) and (F) disease-free survival (p = 0.087) for SMC1A expression in patients with high-grade (stage III and stage IV) CRC (n = 215) is shown.