Figure 4

VEGF treatment induces vWF expression in and secretion from HUVEC cell lines in a dose- and time-dependent manner. HUVECs were exposed to 0, 10, 50 or 100 ng/mL of VEGF for 1 h and examined by western blot (A) and qRT-PCR (C). The concentration of 100 ng/mL induced the highest level of vWF protein and mRNA expression. HUVECs were exposed to the various doses of VEGF for the indicated times and examined by western blot (B) and qRT-PCR (D). The exposure time of 40 minutes stimulated the highest level of vWF protein expression but 80 minutes stimulated the highest level of vWF mRNA expression. *p < 0.05 and **p < 0.01 by Student's t-test.