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Figure 5 | BMC Cancer

Figure 5

From: Multiple myeloma cells alter the senescence phenotype of bone marrow mesenchymal stromal cells under participation of the DLK1-DIO3 genomic region

Figure 5

miR-485-5p influences senescence and cell cycle characteristics of MM-BMMSCs. Asterisks indicate indicate p-values with * <0.05, ** < 0.01, *** < 0.001 and **** < 0.0001. All data were analyzed using the paired t-test. (Ai) Transient overexpression of miR-485-5p led to reduced SA-βGalA whereas inhibition of miR-485-5p increased SA-βGalA. (ii) Representative histograms showing the effect of miR-485-5p inhibitor (n = 5) and miR-485-5p mimic (n = 5) on SA-βGalA in MM-BMMSCs compared to the transfection and negative control. (Bi) In three out of five transfected MM-BMMSCs the inhibition miR-485-5p induced the reduction of cells in S phase and accumulation of cells in G1/G0 phase of the cell cycle. (ii) Representative histogram showing one effect on the cell cycle of MM-BMMSCs induced by the inhibition of miR-485-5p. Reduced amount of cells in S phase is shown. (Ci) Two out of five transfected MM-BMMSCs displayed increased cells in S phase and reduced cells in G1/G0 phase upon transfection with the miR-485-5p inhibitor. (ii) Representative histogram showing the reverse effect on the cell cycle of MM-BMMSCs induced by the inhibition of miR-485-5p. Increased amount of cells in S phase is visible.

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