Figure 4

KMS12-PE myeloma cells downregulate miR-223 and miR-485-5p in MM-BMMSCs. Asterisks indicate indicate p-values with * <0.05, ** < 0.01, *** < 0.001 and **** < 0.0001. All data were analyzed using Wilcoxon signed-rank test. (A) Co-cultured MM-BMMSCs (n = 25) displayed reduced expression of miR-223 and miR-485-5p. Transwell-cultured (n = 10) MM-BMMSCs showed no changes in miR-223 expression but also decreased miR-485-5p levels. Intensity of changes in miR-485-5p decreased when cell-cell-contact was prevented by transwell cultivation. (B) Cell interaction with MM-BMMSCs induced changes in the microRNA expression of KMS12-PE myeloma cells (n = 10). MiR-221 was upregulated whereas miR-223 and miR-519d decreased in co-cultured KMS12-PE myeloma cells. (C) Cell interaction with KMS12-PE myeloma cells led to increased methylation of the IG-DMR of DLK1-DIO3 as measured by qMSP (n = 9).