Figure 2

Overexpressed microRNAs in MM-BMMSCs are associated with hypomethylation and CN accumulation of DLK1-DIO3 and C19MC. Asterisks indicate p-values with * <0.05, ** < 0.01, *** < 0.001 and **** < 0.0001. All data were analyzed using Mann–Whitney U test. (A) ND-MM-BMMSCs (n = 24) and R-MM-BMMSCs (n = 21) showed high overexpression of miR-16, miR-485-5p, miR-519d and miR-223 compared to HD-BMMSCs (n = 10). (B) Schematic presentation of the genomic organization of DLK1-DIO3 on chromosome 14 (C14q32) and C19MC on chromosome 19 (C19q13.41). Circles indicate the regulatory region of the respective cluster and arrows point to positions of CN measurement. Modified from Morales-Prieto et al. [27] (C) The regulatory regions of DLK1-DIO3 and C19MC were hypomethylated in ND-MM-BMMSCs (n = 25) and R-MM-BMMSCs (n = 18) compared to HD-BMMSCs (n = 9). (D) CN analysis of C19MC displayed CN accumulation in all three regions in ND-MM-BMMSCs (n = 23) and R-MM-BMMSCs (n = 15) compared to HD-BMMSCs (n = 8). (E) CN analysis of DLK1-DIO3 displayed CN accumulation in all three measured positions in MM-BMMSCs compared to HD-BMMSCs (sample number as indicated in (D)). No accumulation was measured for R-MM-BMMSCs in position 2.