Figure 8
Follistatin and pSmad2L expression in MDA-MB-436 xenografts is reduced following zoledronic acid treatmentin vivo. A. Representative images of follistatin expression in tumours from saline treated mice at x1.6 magnification (left) and x20 magnification (right). Viable tumour cells (T), necrotic core of tumours (NC). B Representative images of follistatin expression in tumours from ZOL treated mice. C + D. 20 x 750 μm2 images were scored from two sections per tumour. Images were scored for intensity of + ve stain (C) and area of + ve stain (D). Data represents the mean scores + SEM. Mann Whitney U test for significance, ***p value <0.001, NS not significant. E Representative images of pSmad2L expression (black arrows) in saline treated mice (C) and ZOL treated mice (Z). F 20x750μm2 images were scored from two sections per tumour. Number of positive cells were counted and data represents mean scores + SEM. Mann Whitney U test for significance, ***p value <0.001.