Figure 3

Activin and follistatin secretion from ER- breast cancer cell lines and ER + ve breast cancer cell lines. 1x10⁵ MDA-MB-231 or MDA-MB-436 (ER-ve) and 4x10⁵ MCF7 or T47D (ER + ve) cell lines were plated in 6 well plates and levels of Activin (A) and follistatin (B) in the medium determined by ELISA at 24 and 48 hours. Data represents 3 replicates and 3 repeats. Mann Whitney test for significance comparing wells with cells to media alone (no cells), *p value <0.05. <MDL = below assay minimum detection limit.