Figure 5

Changes in the phosphorylation of ERK and AKT after adding fibroblast (HEF -75hTERT) culture supernatant. In representative cell lines (TE-11, TE-14, and KYSE220), we examined the phosphorylation of ERK and AKT six-hours after the addition of supernatant. Both p-ERK and p-AKT levels are markedly up-regulated by treatment with fibroblast supernatant, though neither total AKT nor total ERK are changed. The Y axis indicates the pixel/area.