Figure 4

Effects of cMET inhibition on endothelial cells (ECs) and vascular smooth muscle cells (VSMCs). A) HGF significantly induced motility of EC in vitro (^#P < 0.05); INC280 impaired this effect (*P < 0.05). B) cMET inhibition had no effect on constitutive activation of signaling pathways in ECs. However, HGF strongly induces Akt and ERK phosphorylation which can efficiently be inhibited by INC280. C) Stimulation with HGF had no effect on motility of VSMCs. However, INC280 impaired constitutive motility of these cells in vitro (*P < 0.05). D) In VSMCs, HGF had minor effects on Akt and ERK phosphorylation in vitro. INC280 impairs the effect of HGF, but has no effect on constitutive pathway activation. Bars = SEM.