Figure 7

Analysis of inflammation and desmoplasia on day 20. (A) Representative image of CAE⁺ inflammatory cells in PDA. (B) Quantification of CAE⁺ cells in the carcinomas of sham treated non-diabetic (lean Sham) or diabetic (obese Sham) mice and in the carcinomas of cerulein treated non-diabetic (lean Cer) or diabetic (obese Cer) animals. (C) Desmoplastic reaction visualized by anti-GFP immunohistochemistry in a C57BL6-Tg^{ACTB-eGFP1Osb/J} mouse, which ubiquitously expresses GFP. (D) Quantification of α-smooth muscle⁺ desmoplastic reaction surrounding the carcinomas in sham treated non-diabetic (lean Sham) or diabetic (obese Sham) mice and in the carcinomas of cerulein treated non-diabetic (lean Cer) or diabetic (obese Cer) animals. Box plots indicate the median, the 25^th and 75^th percentiles in the form of a box, and the 10^th and 90^th percentiles as whiskers. The number of animals evaluated was n = 11 (lean Sham), n = 9 (lean Cer), n = 9 (obese Sham), n = 12 (obese Cer) in Panel B and n = 4 for each cohort in Panel D. Bar = 50 μm.