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Figure 6 | BMC Cancer

Figure 6

From: Impact of diabetes type II and chronic inflammation on pancreatic cancer

Figure 6

Analysis of CD133 expression. (A) Analysis of CD133 expression in cultured 6606PDA cells and kidney by Western Blotting. (B) Analysis of CD133 expression in cultured PDA cell lines and kidney by PCR. (C) The positive control for CD133 immunohistochemistry reveals expression of CD133 (arrow) in epithelial cells of proximal tubuli. (D) Immunohistochemistry of 6606PDA derived carcinomas reveals expression of CD133 (arrow) in some cancer cells. (E) Quantification of CD133+ cells in the carcinomas of sham treated non-diabetic (lean Sham) or diabetic (obese Sham) mice and in the carcinomas of cerulein treated non-diabetic (lean Cer) or diabetic (obese Cer) animals. Box plots indicate the median, the 25th and 75th percentiles in the form of a box, and the 10th and 90th percentiles as whiskers. The number of animals evaluated was n = 5 (lean Sham), n = 4 (lean Cer), n = 6 (obese Sham), n = 6 (obese Cer). Differences between the cohorts were not significant. The Western Blot results were reproduced by three independent experiments. Bars = 50 μm.

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