Figure 5

Analysis of CK19, vimentin and Aldh1a1 expression. (A) Representative images of epithelial cells expressing cytokeratin 19 and (B) of non-epithelial cells expressing vimentin in 6606PDA derived carcinomas. (C) Analysis of Aldh1a1 expression in cultured PDA cell lines and kidney by Western Blotting. An additional band (arrow) is observed in some cell lines and kidney cell extract and might represent another Aldh family member; e.g. Aldh1a3. (D) Immunohistochemistry of 6606PDA derived carcinomas reveals expression of the cancer stem cell marker, Aldh1, in some cancer cells. (E) Quantification of Aldh1⁺ cells in the carcinomas of sham treated non-diabetic (lean Sham) or diabetic (obese Sham) mice and in the carcinomas of cerulein treated non-diabetic (lean Cer) or diabetic (obese Cer) animals. Box plots indicate the median, the 25^th and 75^th percentiles in the form of a box, and the 10^th and 90^th percentiles as whiskers. The number of animals evaluated was n = 9 (lean Sham), n = 9 (lean Cer), n = 9 (obese Sham), n = 10 (obese Cer). Significant differences between the cohorts are indicated, *P = 0.003. The Western Blot results were reproduced by three independent experiments. Bars = 50 μm.