Overview of treatments and PacBio results for patients 4 and 5. A) Results for patient 4. The BCR-ABL1 IS% values measured by routine quantitative RT-PCR are shown in open circles. The sensitivity of this assay was measured for the BGUS reference gene and depicted by gray squares. As indicated by the red cross, the T315I mutation was detected after 58 months of dasatinib treatment. The mutation was detected at this time point using our allele specific quantitative PCR used in routine analysis. The samples that were analyzed by PacBio sequencing are indicated by black arrows and their mutation composition showed in the circle plot diagrams. Vertical lines indicate the treatment periods. HU (Hydroxyurea). B) Results for patient 5. Measurements shown were made as in A. AlloSCT: allogeneic stem cell transplantation. DLI: donor lymphocyte infusion.