Figure 3

Overview of treatments and PacBio results for patients with single mutations. A) Results for patient 1. The BCR-ABL1 IS% values measured by routine quantitative RT-PCR are shown in open circles. The sensitivity of this assay was measured for the BGUS reference gene and depicted by gray squares. The samples that were analyzed by PacBio sequencing are indicated by black arrows and their mutation composition showed in the circle plot diagrams above each time point. Vertical lines indicate the treatment periods. HU (Hydroxyurea). B) Results for patient 2. The T315I mutation was detected after nilotinib treatment, as indicated by the red cross. The mutation was detected at this time point using our allele specific quantitative PCR used in routine analysis.