BCR-ABL1mutations and their composition in patient samples. Overview of BCR-ABL1 mutations detected in five CML patients (P1-P5) at the time of diagnosis and at subsequent follow-up examinations following TKI treatment. Samples taken at the time of diagnosis are labeled ‘Diag’. The follow-up samples are labeled with the number of months after diagnosis. The numbers next to the colored bars show the frequencies of all mutations observed by PacBio sequencing. Asterisks (*) indicate mutations that failed to be detected by Sanger sequencing.