Figure 3
From: Enhanced tumour cell nuclear targeting in a tumour progression model
Engineered histone H3 proteins bind to DNA. The indicated recombinant proteins were incubated at increasing concentrations (left to right; 0.1, 0.25, 0.5, 0.75 and 1 μM for the histone proteins and 0.5 and 1 μM for GFP) with 300 ng of linearised plasmid DNA (6.7 KB) for 30 min at room temperature prior to electrophoresis (40 V) at 4°C for 16 h against DNA alone (−). GFP alone was also incubated with DNA in the same way. Results are from a single typical experiment from a series of two similar experiments.