Figure 5

S100A4(-/-) mice have smaller CD4⁺and CD8⁺populations in the spleen. (A) Representative pictures of the flow-cytometry analysis of CD4⁺ and CD8⁺ T-cells from the spleen of S100A4(+/+) and S100A4(-/-) mice. Shown are the average percentages of CD4⁺ and CD8⁺ lymphocytes in the spleen (n = 12). (B) The absolute number of CD4⁺ and CD8⁺ lymphocytes in the spleen of S100A4(+/+) and S100A4(-/-) mice (n = 12) determined by flow-cytometry. (C) (i) Cell viability (LDH assay) of primary T-lymphocytes expanded with or without S100A4 protein. The LDH activity is measured at days 1, 2, 3 and 5. Proliferation rate of primary T-lymphocytes expanded with or without S100A4 protein. (ii) The number of cells is measured at days 1, 2, 3, 5 and 6.