S100A4 shifts the Th1/Th2 balance of differentiating T-cells in vitro. (A) The percentage of CD4+ T-cells in S100A4-treated and control T-cell populations at 3 and 6 days. (B) Representative pictures of the flow cytometry analysis of primary Th1/Th2 polarized CD4+ T-cells. Primary T-cells were expanded with or without S100A4 protein for 6 days. (C) The percentage of Th1-polarized CD4+ T-cells in the S100A4-treated and control populations. (D) The relative ratio of Th1/Th2 polarized T-cells after stimulation with S100A4 protein for 3 and 6 days. (E) The relative ratio of Th1/Th2 cells after stimulation of T-cells for 6 days with the S100A4 protein or with S100A4 protein mixed with 6B12. (F) Determination of IFNγ and IL10 levels in CM from T-cells expanded with or without S100A4 protein for 6 days by Sandwich ELISA. Data are in triplicate. ***P < 0.001.