Effect of miR-130b on the proliferation of ESCC cellsin vitro. The viability of Eca109 and TE13 cells transfected with miR-130b mimic, negative control, miR-130b inhibitor, inhibitor negative control or siPTEN was detected by CCK-8 at 24, 48, 72 and 96 h. The absorbance was measured at 450 nm. (A, B) Proliferation curves of Eca109 cells. (C, D) Proliferation curves of TE13 cells. For colony formation (E, G) and soft-agar (F, H) assays in Eca109 cells, the following calculation was performed: colony-forming efficiency (%) = the number of colonies (>50 cells or larger than 50 μm)/the number of cells plated per well. The photographs were captured from digital camera or microscope at 40× magnification, in which the size bar was representative of 200 μm. The results are expressed as mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01 vs. corresponding controls; #P < 0.05, ##P < 0.01 vs. 130bi group.