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Figure 3 | BMC Cancer

Figure 3

From: MiR-130b plays an oncogenic role by repressing PTEN expression in esophageal squamous cell carcinoma cells

Figure 3

MiR-130b regulates the expression of PTEN and phosphorylation of Akt. Eca109 and TE13 cells were transfected with miR-130b mimic (130bm group) or inhibitor (130bi group); siPTEN was also co-transfected with the miR-130b inhibitor to decrease PTEN expression (130bi + siPTEN group). Protein expression of PTEN, p-Akt and total Akt in Eca109 (A, C, E) and TE13 cells (B, D, F) was determined by Western blot analysis. PTEN mRNA expression was detected by SYBR Green real-time PCR at 24 h following transfection of Eca109 (G) and TE13 (H) cell lines. The values were normalized to the corresponding controls. GAPDH was the endogenous control. *P < 0.05, **P < 0.01 vs. corresponding controls; #P < 0.05 vs. 130bi group.

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