Identification of PTEN as target of miR-130b. (A) Bioinformatic prediction by TargetScan indicated that PTEN mRNA contains a putative miR-130b binding region, located at 412–418 nt of 3′-UTR. (B) A dual-luciferase reporter assay was used to confirm the interaction of miR-130b with PTEN. A fragment of miR-130b that was predicted to bind to the PTEN 3′-UTR was cloned into firefly luciferase pGL3-control vector. The Renilla luciferase plasmid was co-transfected for normalizing luciferase activity. *P < 0.05, **P < 0.01 vs. corresponding controls.