Figure 3
HSC-3 monocultures with two types of triple immunohistochemical panels. Caveolin-1 (CAV1), Twist and cytokeratin (CK) in rinsed and intact myoma in normoxia and hypoxia [cobalt chloride (CoCl2) or in hypoxia chamber with 1% O2)] (a-f) show similar patterns of expression in all settings: positive nuclear staining of the invading HSC-3 islands with Twist (most nuclei) and diffuse cytoplasmic staining with cytokeratin and, to a lesser extent, with CAV1 (arrows). In addition, delicate, spindle-shaped cells that closely surround these invading tumor islands in a concentric pattern are also CAV1-positive. CK and alpha-smooth muscle actin (αSMA) in rinsed and intact myoma in normoxia and hypoxia [cobalt chloride (CoCl2) or 1%O2)] (g-l) show similar patterns of expression in all settings: positive nuclear staining of the invading HSC-3 islands with Twist (most nuclei) and diffuse cytoplasmic staining with CK. In addition, delicate, spindle-shaped cells that closely surround these invading tumor islands in a concentric pattern are αSMA-positive.