EphA5 overexpression inhibited the migration and invasiveness of Du145 cells. A. Western blot analysis of EphA5 protein (114 kDa band) expression in DU145 cells after transfection with pCMV6-GFP-EphA5. B. Cell migration activity determined with the wound healing assay. C. Cell invasion activity determined with the Matrigel invasion assay (×100). All experiments were performed in triplicate. Data are shown as the mean ± SD. *P < 0.05 vs. pCMV6-AC-GFP group.