Figure 5

NO-induced up-regulation of PVR/CD155 is not related to a senescence-dependent mechanism. A) PVR/CD155 surface expression was analyzed by flow cytometry on SKO-007(J3) cells treated with DETA-NO (200 μM) or with doxorubicin (0.05 μM) for 48 h. Data are representative of one out of three independent experiments. The grey colored histograms represent basal expression of the indicated ligand, while thick black colored histograms represent the expression after treatment with the indicated drug. B) SA-βGal activity of SKO-007(J3) cells treated with DETA-NO or with doxorubicin for 48 h as described above. Data are representative of one out of three independent experiments. The grey colored histograms represent the C12-fluorescein signal. C) SKO-007(J3) cells were treated for 48 hours with the indicated drug as described above. Cells were fixed and stained with PI to analyze cell distribution among the different cell-cycle phases.