Figure 5From: Dermcidin exerts its oncogenic effects in breast cancer via modulation of ERBB signalingSignaling pathways modulated by DCD. A, Expression of ERBB family of receptors and their ligands (B) in control and DCD shRNA-expressing cells. Y-axis indicates mean ± SD mRNA levels (normalized to HPRT) relative to pLKO controls. C, Proliferation-inducing activity of highly purified recombinant human DCD in MDA-MB-361 based on BrdU incorporation assay. Columns indicate mean of absorbance units, bars, SD. Real time PCR analyses of EGFR (D), C-myc (E), ERBB ligands (F) and ERBB receptors (G) at the indicated doses of rhDCD. Each sample was analyzed in three independent experiments performed in duplicates. * and ** denotes P < 0.05 and P <0.01, respectively, by Student’s t test. H, effects of EGF treatment on the phoshorylation of EGFR and downstream signaling proteins p38-MAPK and pAkt in MDA-MB-361 control and DCD shRNA-expressing following of treatment with EGF 10 ng/ml for 15 minutes.Back to article page