Figure 4

The effect of DCD levels on global gene expression profiles. A, Heatmap depicting relatedness of gene expression profiles of control and DCD shRNA expressing cells. Hierarchical clustering was applied to Microarray data and selected portions of the clustering heat map are shown here. Each row represents a probe and the official symbol of gene is shown. Red and green indicate high and low gene expression levels, respectively. B, Gene ontology biological process categories highly represented in DCD shRNA expressing cells. Categories with an enrichment score >2 using the DAVID Functional Annotation Tool are plotted. C, Development_ERBB signaling canonical pathway map generated by MetaCore. Blue thermometers in Betacellulin, amphiregulin, EGFR and c-Myc indicate fold-change in expression levels in each of the three different DCD shRNA expressing cell pools compared to control pLKO. See Additional file 5 for network legend.