Figure 3

The effect of DCD downregulation on tumorigenesis. A, Representative mice from control and DCD shRNA experimental groups twelve weeks after injection demonstrating visible differences in tumor size. No physical signs resulting from cachexia were observed in either group of mice. B, Body weight of mice during the course of the experiment. Data are mean ± SE for 5–6 mice per group. *indicates statistically significant (P < 0.05) differences calculated by ANOVA. C, Variation of body weight along xenograft tumor growth. Data are expressed as mean ± SE of the average of weights of 5–6 animals in each experimental group. Statistical differences among groups were determined by One-way ANOVA with Turkey’s pairwise comparisons. D, Immunohistochemical analysis of DCD and cytokeratin 5 (CK-5) and 18 (CK-18) protein expression in xenografts derived from cells expressing DCD shRNA (IBC-I) compared to pLKO controls. E, Table 1 showing summary of tumor size and weight of carcass, skeletal muscles, and individual organs. Data are expressed as mean ± SE of the average of wet weights of organs of 5–6 animals in each experimental group. Statistical differences among groups were determined by One-way ANOVA with Turkey’s pairwise comparisons.