TOX3 enhances MCF-7 migration and is upregulated by IGF-1. A. qRT-PCR and B. FACS analysis, for CXCR4 expression in two independently generated vector or TOX3 stably transfected MCF-7 cell lines. C. Representative images of migratory cells in vector control (V) or TOX3-expressing (TOX3) cell lines. D. Quantification of migratory cells in vector and TOX3 transfected cell lines using FBS as chemo-attractant. Data are expressed as mean ± SD of three independent experiments. E. qRT-PCR analysis of TOX3 and GAPDH (a positive control for IGF-1 response), relative to ACTB, in MCF-7 cells treated with IGF-1. Data are expressed as mean ± SD of three independent experiments.