Expression of TOX3 in MCF-7 cells leads to upregulation of genes implicated in cancer progression. A. Two-way hierarchical clustering of gene expression changes in MCF-7 cells upon transient expression of TOX3. B. qRT-PCR validation of selected genes from microarray result. Data are expressed as mean ± SD of three independent experiments. C. TOX3 mRNA and protein expression (inset) in MCF-7 stable cell lines. D. Cell survival of stably transfected MCF-7 cell lines at indicated days, grown in estrogen-depleted medium. Data are expressed as mean ± SD of three cultures. E. BT474 cell growth upon siRNA-mediated knockdown of TOX3. Data are expressed as mean ± SD of five independent experiments.