Development of a semi-conductor sequencing-based panel for genotyping of colon and lung cancer by the Onconetwork consortium

Table 2 Variants identified in the 5 control samples

Sample	Known mutations	Mutations identified by NGS^‡	Allele frequency^#
L1	KRAS: p.Gly12Cys	KRAS: p.Gly12Cys	0.44 - 0.54
A12	KRAS: p.Gly12Ala	KRAS: p.Gly12Ala	0.66 - 0.67
		FBXW7: p.His460Tyr	0.46 - 0.49
		TP53: p.Ala159Asp	0.74 - 0.75
A13	KRAS: Gly13Asp	KRAS: Gly13Asp	0.50 - 0.56
		PIK3CA: p.Asp549Asn	0.43 - 0.46
		DDR2: p.Thr98Ala	0.47 - 0.56
		FGFR1: p.Ala268Ser	0.50 - 0.66
		NOTCH1: p.Pro1581Leu	0.48 - 0.54
X23	KRAS: p.Gly12Asp	KRAS: p.Gly12Asp	0.65 - 0.75
	PIK3CA: p.Glu545Lys	PIK3CA: p.Glu545Lys	0.40 - 0.51
X32	KRAS: p.Gly12Asp	KRAS: p.Gly12Asp	0.54 - 0.69
	PIK3CA: p.Glu542Lys	PIK3CA: p.Glu542Lys	0.44 - 0.46
	FBXW7: p.Arg465His	FBXW7: p.Arg465His	0.46 - 0.55
	TP53: p.Gly244Asp	TP53: p.Gly244Asp	0.32 - 0.35

^‡Newly identified variants were verified by conventional Sanger sequencing.
^#Indicated is the range of the allele frequencies over the different laboratories.

ISSN: 1471-2407