STK33 overexpression in hypopharyngeal squamous cell carcinoma: possible role in tumorigenesis

BMC Cancer

Table 2 Primer, amplicon size and annealing temperature of each gene for qRT-PCR

Gene	Forward 5′to3′	Reverse 5′to3′	Product length/bp	Tm/°C	Genbank NCBI reference sequence
STK33	CCCCGACTGTTCATCTGCTTCT	TGCCCACTTCGTTTCTGTTTCC	391	54	Nm_030906.2
ERK1/2	TCCAAGGGCTACACCAAGTC	CTCGTCACTCGGGTCGTAAT	363	60	Nm_002745.4
Caspase-3	CAGAACTGGACTGTGGCATTG	GCTTGTCGGCATACTGTTTCA	192	54	Nm_004346.3
Bcl-2	GTGGCCTTCTTTGAGTTCGG	TCCACAGGGCGATGTTGTC	88	55	Nm_000633.2
Ki-67	TGTCACATCGCTATTTCAAATTCAG	GGAGGGCTTGCAGAGCATTTATC	170	54	Nm_001145966.1
E-Cadherin	GGGGTCTGTCATGGAAGGTGCT	GTAAGCGATGGCGGCATTGTAG	103	57	Nm_004360.3
Vimentin	AGGCAGAAGAATGGTACAAATCC	TTTAAGGGCATCCACTTCACAG	146	55	Nm_003380.3
Nm-23-H1	CTGGGACCATCCGTGGAGACT	TCCTCAGGGTGAAACCACAAGC	112	55	Nm_000269.2
GAPDH	AGGTCGGTGTGAACGGATTTG	TGTAGACCATGTAGTTGAGGTCA	130	55	Nm_001289746.1

ISSN: 1471-2407