Figure 1

Schematic overview of model patterning methods. As previously described, a lumen can be patterned through a collagen I hydrogel in the center chamber of a triple microchannel using viscous finger patterning [22]. The collagen I lumen is then coated with a Matrigel lining. Following polymerization, MCF10a cells are used to line the lumen and mimic a mammary duct structure. HMF cells in a collagen I hydrogel can be added to the side chambers. To model DCIS, MCF10aDCIS.com cells are flowed into the lumen after 24 hours.