Figure 3
Effect of BPs on the response to LPS in 4T1CM-treated J774 cells. A mRNA expression of iNOS, IL-6, IL-12, and MMP-9. B Protein levels of M-CSF, VEGF, TNF-α, IL-10, MCP-1 and IL-6 secreted by macrophages. *p < 0.05, **p < 0.005, student’s t-test as compared with the CTR and EMP-LIP (* lines above the bars). C Nitrite (NO2-) production. *p < 0.05, one-way ANOVA with Dunnett’s multiple comparison test as compared with the CTR. A-C Data represent the means ± SEM of 4 independent experiments. D Zymography analysis of pro-MMP-9 in J774 cell supernatants. Presented values are the optical densities of pro-MMP-9-digested bands normalised to the total protein content of the corresponding total cell lysate compared with appropriate controls (CTR or EMP-LIP). E IPP, ApppI and AppCCl2p levels after treatment. Data represent the means ± SEM of three independent experiments. ***p < 0.0001, one-way ANOVA with Dunnett’s multiple comparison test as compared with the CTR.