Figure 1
Different mRNA and secreted cytokine profiles of 4T1 and 3T3 cells lead to differences in the modulation of J774 cell activation status with or without LPS stimulus. A mRNA expression levels of iNOS, IL-6, IL-12, and MMP-9. B Protein levels of M-CSF, VEGF, TNF-α, IL-10, MCP-1 and IL-6 secreted by macrophages. C Nitrite (NO2-) production. Data represent the means ± SEM of 5 independent experiments. *p < 0.05, one-way ANOVA with Dunnett’s multiple comparison test as compared with the CTR. D Zymography analysis of pro-MMP-9 in cell supernatants . Data represent the means ± SEM of four independent experiments. *p < 0.05, **p < 0.005, student’s t-test as compared with the CTR or the CTR + LPS treatment (* lines above the bars). E-G Data represent the means ± SEM of 3 independent 72-h post-confluence cultures assayed in duplicate. E Mean mRNA expression levels of iNOS, IL-6, IL-12, and MMP-9 normalised to GAPDH. F Secreted protein levels of M-CSF, VEGF, TNF-α, IL-10, MCP-1 and IL-6. G Zymography analysis of pro-MMP-9 in cell supernatants.