Figure 3

Curcumin and its analogues, GO-Y030, FLLL-11, and FLLL-12 induced PARP and/or caspase-3 cleavages in (A) HCT116, (B) HT-29, (C) SW480 human colorectal cancer cells, (D) WI-38 and MCF-10A cells, (E) normal human and colonic bladder smooth muscle cells. Colorectal cancer cells and normal human cells were treated for 24 hours. Western blot was used to detect the PARP cleavages using antibodies against cleaved PARP and cleaved caspase-3 (Cell Signaling Technology, Beverly, MA). The same membranes were analyzed with a 1:1000 dilution of anti-GAPDH monoclonal antibody (Chemicon International, Inc., Temecula, CA).