Table 1 MRM transition chart for the identification of putative protein biomarkers.
Protein/Peptides | z | -10lg(p) | Spectral count | MRM range (min) | Transitions (MH+ → product ions) |
|---|---|---|---|---|---|
O43399|TPD54_Human Tumor Protein D54 | |||||
GLLSDSMTDVPVDTGVAAR | 2 | 91 | 2 | 40-90 | 952.47→(885.3, 1019.2, 1200.3, 1099.2, 574.3) |
VVGDRENGSDNLPSSAGSGDKPLSDPAPF (no ID) | 3 | 136 | 15 | 0-240 | 962.45→(1312.0, 1228.1, 1127.0, 1256.3, 815.9) |
LGLSTLGELKQNLSR (no ID) | 2 | 114 | 5 | 0-240 | 814.97→(1044.3, 617.3, 745.3, 673.4, 1157.4) |
TPAVEGLTEAEEEELRAELTKVEEEIVTLR | 3 | 300 | 8 | 145-170 | 1128.58→(1508.1, 1443.7, 1358.6, 1063.0, 1244.1) |
P31947|1433S_Human 14-3-3 protein sigma | |||||
LAEQAERYEDMAAFMK | 2 | 112 | 4 | 40-90 | 951.94→(1205.2, 698.1, 1478.2, 795.4, 1625.2) |
VLSSIEQKSNEEGSEEKGPEVR (no ID) | 3 | 133 | 9 | 0-240 | 811.06→(740.6, 1110.0, 966.5, 1080.0, 557.2) |
P27797|CALR_Human Calreticulin precursor | |||||
SGTIFDNFLITNDEAYAEEFGNETWGVTK | 3 | 300 | 25 | 125-145 | 1090.16→(1512.0, 1424.0, 1462.1, 991.2,1289.7) |
FYGDEEKDKGLQTSQDAR | 2 | 141 | 13 | 0-40 | 1043.98→(1841.9, 803.6, 975.5, 1112.6, 1104.6) |
IDNSQVESGSLEDDWDFLPPKK | 3 | 85 | 14 | 0-240 | 840.40→(981.9, 1146.5, 932.8, 1089.3, 1204.0) |
P08195|4F2_Human 4F2 cell-surface antigen heavy chain | |||||
ADLLLSTQPGREEGSPLELER | 3 | 84 | 5 | 40-90 | 770.73→(706.2, 893.3, 900.6, 842.4, 1004.3) |
IKVAEDEAEAAAAAK | 2 | 94 | 5 | 0-240 | 743.89→(1245.2, 1146.1, 946.1, 831.1, 1075.1) |
P07339|CATD_Human Cathepsin D precursor | |||||
ISVNNVLPVFDNLMQQK | 2 | 119 | 22 | 90-125 | 980.02→(1219.3, 1332.3, 880.5, 610.1, 724.2) |
LVDQNIFSFYLSRDPDAQPGGELMLGGTDSK | 3 | 300 | 23 | 0-240 | 1124.21→(1261.3, 1344.6, 1055.2, 1016.1, 1579.6) |
P46013|Ki-67 antigen (no ID) | |||||
AQALEDLAGFKELFQTPGHTEELVAAGK (no ID) | 3 | 71 | 1 | 0-240 | 990.84→(1208.4, 1080.1, 604.9, 1386.6, 924.7) |
SGGSGHAVAEPASPEQELDQNKGK (no ID) | 3 | 44 | 1 | 0-240 | 798.38→(956.0,870.9, 643,2, 921.0, 653.1) |
P12004|PCNA_HUMAN Proliferating cell nuclear antigen (no ID) | |||||
ATPLSSTVTLSMSADVPLVVEYK (no ID) | 2 | 106 | 4 | 0-240 | 1204.63→(847.2, 1561.2, 1061.2, 1437.3, 538.1) |
FSASGELGNGNIK (no ID) | 2 | 98 | 7 | 0-240 | 647.32→(716.1, 744.2, 546.1, 902.2, 989.2) |
LSQTSNVDKEEEAVTIEMNEPVQLTFALR (no ID) | 3 | 101 | 5 | 0-240 | 1097.88→(1559.1, 1551.3, 1393.6, 1467.1, 937.4) |
YYLAPKIEDEEGS (no ID) | 2 | 112 | 8 | 0-240 | 757.35→(1003.5, 676.4, 511.2, 1074.5, 1187.6) |
DLSHIGDAVVISCAK (no ID) | 2 | 113 | 4 | 0-240 | 764.39→(962.2, 566.1, 606.9, 650.4, 1075.2) |
NLAMGVNLTSMSK (no ID) | 2 | 35 | 5 | 0-240 | 683.34→(503.2, 864.1, 1064.1, 1067.3, 936.2) |