PI3K and beta1-integrin differently regulate staged 3D ECM induced MDA-MB-231 morphologies. MDA-MB-231 cells were cultured overnight on 2D or within staged 3D ECMs, control and tumor-associated matrices, in the presence and/or absence of 10 and 50 nM Wortmannin and/or 50 μg/ml mAb13. a. Individually scattered dots represent elliptical form factors (EFF). Upper and lower borders of diamond shapes show 75 and 25 percentile EFF populations, respectively while diamond widths marked with a horizontal line denote median EFF values. b. Representative transmitted light micrographs depicting one cell for each stated experimental condition. Scale bar indicates 50 μm. Note that median EFF increases in response to tumor-associated 3D ECMs, and that these EFFs can be inhibited by high Wortmannin or mAb13 treatments while combinations of both inhibitors seemed to inhibit better than each of these alone. See Table 1 for detailed quantitative data, Table 2 for statistical information and Additional Files 4, 5 and 6 (Movies 4–6) for additional examples.