Figure 4

Effects of flutamide administration and castration on the radiation- induced injury by immunochemical analysis. Immunohistochemical analyses using an antibody against TGF-β (Magnification ×400) were performed on lung (A) and intestinal (B) tissues. Three mice from each group were examined. Representative slides are shown for (a) unirradiated control mice, (b) castrated mice without irradiation, (c) irradiated mice at 24 h after 20 Gy irradiation, (d) flutamide-treated mice 24 h after 20 Gy irradiation, (e) castrated mice 24 h after 20 Gy irradiation and (f) Lupron-treated mice 24 h after 20 Gy irradiation. Increased TGF-β immunoreactivity was detected 24 h after irradiation. Castration augmented the inflammatory response, whereas androgen deprivation by Flutamide administration had no significant effect. Duplicate experiments were performed for the analysis. (C) Expression of TGF-β1 protein in irradiated murine intestinal and lung tissue with or without androgen deprivation including castration and flutamide administration. (a, control; b, irradiation; c, castration alone; d, castration plus irradiation; e, flutamide administration; f, irradiation plus flutamide administration). (Y axis represents the relative protein level which is normalized by the protein level of TGF-β1 in control condition).