Figure 2

Effect of androgen deprivation including flutamide and castration on pro-inflammatory cytokines in lung, liver and intestinal tissues. (A-C) The mRNA levels of the cytokines TNF-α, IL-6, IL-1α/β and TGF-β were quantified by real-time RT-PCR. The results were normalized to the value of irradiated mice. The y-axis shows the RNA ratio of each target gene in lung (A), liver (B) and intestinal (C) tissue (control and irradiated mice with flutamide administration) divided by that in the irradiated mice (without flutamide administration). (D) Effect of flutamide on the MPO activities in lung, liver and intestinal tissues. The y-axis shows the ratio of MPO activity in irradiated tissues with flutamide administration or castration divided by irradiated tissues without androgen deprivation. Data are the mean ± SE. Columns, means of 3 separate experiments; bars, SD. * P < 0.05